Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

Table 1 MS parameters on the precursor and product ion transitions for signature peptides, isotope-labeled signature peptides and internal standard peptides

Protein	Name	Sequence	Precursor ion (m/z)	Q1 mass (amu)	Q3 mass (amu)	DP (V)	CE (V)	CXP (V)	EP (V)
OsGSTF14	Signature peptide 1	VFGSPTSAEVAR	[M + 2H]²⁺	610.9	830.4	84.20	28.98	9.69	10
	Isotope-labeled signature peptide 1	VFGSPTSAEV*AR	[M + 2H]²⁺	613.9	836.4	95.97	29.30	9.46	10
	Internal standard peptide 1	APASVKVFGSPTSAEV*ARVLMCLF	[M + 3H]³⁺	829.7	987.6	106.17	28.84	7.46	10
OsGSTU6	Signature peptide 2	TPLLAAWAER	[M + 2H]²⁺	564.3	703.3	83.98	29.74	6.85	10
	Isotope-labeled signature peptide 2	TPLLAAWAER*	[M + 2H]²⁺	569.3	713.3	104.65	30.15	6.85	10
	Internal standard peptide 2	LVDAGKTPLLAAWAER*FVEVEA	[M + 3H]³⁺	799.3	1039.8	89.30	27.19	6.90	10

DP declustering potential, CE collision energy, CXP collision cell exit potential, EP entrance potential

ISSN: 1746-4811