Skip to main content
Fig. 6 | Plant Methods

Fig. 6

From: Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach

Fig. 6

The silencing of off-targets in TRV-UBC12/10/28/31-infected N. benthamiana plants. a The expression level of group III E2 genes in TRV-UBC12/10/28/31-infected N. benthamiana plants. The transcript level of group III E2 genes in non-silenced TRV control (TRV) and TRV-UBC12/10/28/31-infected N. benthamiana plants was examined by semi-quantitative PCR. NbEF1α was used as an internal reference for determining the amount of cDNA template to be used. Numbers under the gel bands denote the percentage of reduction (%) in the expression of corresponding gene in TRV-III-infected plants compared to that in non-silenced TRV control plants. b Phenotype of the N. benthamiana plants in TRV-UBC12/10/28/31-infected N. benthamiana plants. The non-silenced TRV empty-vector (TRV) and group III E2-silenced plants were used as control. Photographs were taken 4 weeks after the approximately 3-week old seedlings were infiltrated with the TRV-UBC12/10/28/31 construct, the TRV-III construct, and the TRV empty vector, respectively. The experiment was repeated two times with similar result

Back to article page

Plant Methods

ISSN: 1746-4811