Table 2 List of identified mutants
From: Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
Targetedgenes | Accessiona | Description | Function category | Insertion locib |
|---|---|---|---|---|
POC2 | Cre10.g436000 | Polycystin-related | Transition zone | R, 2nd intron; F, 1st exon |
CRX | Cre03.g202950 | Ef-hand calcium-binding domain containing protein | Calredoxin | R, 2nd intron |
MAN1 | Cre07.g336600 | Alpha-1,2-mannosidase | Glycosylation | R, 7th exon |
CUL3 | Cre17.g734400 | Ubiquitin ligase SCF complex subunit Cullin | Ubiquitin ligase | R, 3′UTR |
XYLT | Cre09.g391282 | Glycoprotein 2-beta-d-xylosyltransferase | Glycosylation | F, 6th intron; F, 7th intron |
PKD2 | Cre17.g715300 | Polycystin cation channel protein 2 | Flagellar protein | F, 11th exon; F, 3rd exon |
AMYB1 | Cre06.g307150 | Beta-amylase | Starch metabolism | R, 5′UTR; R, 3′UTR (two different mutants) |
SSS2 | Cre03.g185250 | Soluble starch synthase II | Starch metabolism | R, 1st intron |
FAP215 | Cre03.g180450 | 5′-nucleotidase and flagellar associated protein | Flagellar protein | R, 13th intron |
FAP208 | Cre11.g482001 | Ankyrin repeat-containing protein | Flagellar protein | F, 22nd exon |
FAP249 | Cre17.g746697 | Flagellar associated protein | Flagellar protein | R, 5′UTR (ligated with 480 bp unknown sequence) |
FAP164 | Cre17.g735350 | Flagellar associated protein | Flagellar protein | F, 2nd intron |
CDPK3 | Cre01.g009500 | Protein kinase domain (Pkinase) | Flagellar protein | F, 2nd intron (ligated with partial left border of insertion fragments) |
PDCD6 | Cre03.g200050 | Ca2+Â -binding protein, EF-hand protein superfamily | ESCRT | R, 2nd intron; R, 4th intron |
VPS4 | Cre02.g079300 | AAA-ATPase of VPS4/SKD1 family | ESCRT | R, 3′UTR |
VPS37 | Cre08.g362550 | Subunit of the ESCRT-I complex | ESCRT | F, 2nd intron |
PEPC2 | Cre03.g171950 | Phosphoenolpyruvate carboxylase | Lipid metabolism | R, 19th exon |
IFT139 | Cre06.g268800 | Intraflagellar transport protein 139 | IFT | R, 22nd intron |
UBA1 | Cre09.g386400 | Ubiquitin-activating enzyme E1 | Ubiquitin | R, 3′UTR |
FRE2 | Cre05.g241400 | Ferric-chelate reductase (NADH) | Ferric protein | R, 5′UTR |