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Fig. 6 | Plant Methods

Fig. 6

From: A novel hairpin library-based approach to identify NBS–LRR genes required for effector-triggered hypersensitive response in Nicotiana benthamiana

Fig. 6

TMV-induced hypersensitive response (HR) in N-infiltrated leaves is cancelled by NRG1 silencing. Hairpins hp#26 or u135 or the corresponding empty vector were co-infiltrated with N in N. benthamiana leaves and the infiltrated areas were marked with a felt-tip pen. TMV was inoculated 24 h after Agrobacterium infiltration. HR was visible from 4 days after TMV inoculation and photo were taken 6 days post-inoculation. H2O2 production in the same leaves was monitored using DAB staining

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