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Fig. 4 | Plant Methods

Fig. 4

From: A novel hairpin library-based approach to identify NBS–LRR genes required for effector-triggered hypersensitive response in Nicotiana benthamiana

Fig. 4

Pto/avrPto-triggered HR is cancelled by hp#12 pre-infiltration. a General design of the assay: N. benthamiana leaf was infiltrated with Agrobacterium containing the hairpin construct or corresponding empty vector (EV) in two distinct patches on the right-hand side and left hand side of the leaf respectively and infiltration areas were marked with a felt-tip pen. The following day, Agrobacterium containing the effector construct, or corresponding empty vector (EV), were infiltrated in the pre-infiltrated patches of the top half and bottom half of the leaf respectively. Development of the HR was monitored during the following 3–7 days depending on the strength of the HR. b Agrobacterium suspension (OD 0.2) carrying the hairpin hp#12 or corresponding empty vector was infiltrated in N. benthamiana leaf and the Agrobacterium suspension (OD 0.2) expressing the Pto/avrPto construct was infiltrated 24 h later on the pre-infiltrated patches as designed in (a). Photos was taken 4 days after Pto/avrPto infiltration

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Plant Methods

ISSN: 1746-4811