Table 3 Analyses of reference genes evaluated according to NormFinder and RefFinder algorithms
From: Reference genes for normalization of qPCR assays in sugarcane plants under water deficit
Experimental condition | NormFinder | RefFinder | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
NormFinder | geNorm | BestKeeper | DeltaCt | |||||||
Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | |
Field | UBQ1 | 0.271 | UBQ1 | 0.365 | ACT/RPL | 0 | GAPDH | 0.539 | UBQ1 | 0.770 |
ACT | 0.350 | 25SrRNA2 | 0.494 | UBQ2 | 0.559 | ACT | 0.591 | 25SrRNA2 | 0.820 | |
25SrRNA2 | 0.351 | TUB | 0.620 | UBQ1 | 0.713 | RPL | 0.591 | RPL | 0.860 | |
RPL | 0.358 | UBQ2 | 0.629 | GAPDH | 0.759 | UBQ1 | 0.617 | ACT | 0.860 | |
UBQ2 | 0.367 | GAPDH | 0.640 | TUB | 0.769 | 25SrRNA2 | 0.630 | GAPDH | 0.880 | |
TUB | 0.404 | RPL | 0.663 | 25SrRNA2 | 0.800 | TUB | 0.639 | TUB | 0.880 | |
GAPDH | 0.418 | ACT | 0.663 | 25SrRNA1 | 0.892 | UBQ2 | 0.663 | UBQ2 | 0.880 | |
25SrRNA1 | 0.525 | 25SrRNA1 | 1.054 | 25SrRNA1 | 0.930 | 25SrRNA1 | 1.170 | |||
Best pair | UBQ1/ACT | 0.164 | ||||||||
Greenhouse | GAPDH | 0.304 | GAPDH | 0.424 | ACT/RPL | 0 | GAPDH | 0.438 | GAPDH | 0.840 |
UBQ2 | 0.335 | UBQ2 | 0.565 | UBQ2 | 0.419 | UBQ2 | 0.525 | UBQ2 | 0.890 | |
ACT | 0.344 | TUB | 0.673 | GAPDH | 0.577 | RPL | 0.543 | ACT | 0.920 | |
RPL | 0.355 | UBQ1 | 0.739 | TUB | 0.729 | ACT | 0.543 | RPL | 0.920 | |
TUB | 0.419 | RPL | 0.741 | UBQ1 | 0.824 | TUB | 0.607 | TUB | 0.970 | |
UBQ1 | 0.439 | ACT | 0.741 | 25SrRNA2 | 0.920 | 25SrRNA1 | 0.630 | UBQ1 | 1.010 | |
25SrRNA1 | 0.462 | 25SrRNA2 | 0.831 | 25SrRNA1 | 0.957 | 25SrRNA2 | 0.637 | 25SrRNA2 | 1.030 | |
25SrRNA2 | 0.507 | 25SrRNA1 | 0.902 | UBQ1 | 0.675 | 25SrRNA1 | 1.070 | |||
Best pair | 25SrRNA1/UBQ2 | 0.211 | ||||||||
Hydropony—PEG8000 | GAPDH | 0.150 | GAPDH | 0.239 | GAPDH/ACT | 0.477 | UBQ1 | 0.750 | GAPDH | 0.65 |
ACT | 0.292 | ACT | 0.572 | RPL | 0.609 | GAPDH | 0.792 | ACT | 0.82 | |
RPL | 0.362 | RPL | 0.716 | UBQ1 | 0.774 | RPL | 0.848 | RPL | 0.91 | |
UBQ1 | 0.407 | UBQ1 | 0.729 | UBQ2 | 0.859 | ACT | 1.018 | UBQ1 | 0.93 | |
UBQ2 | 0.466 | UBQ2 | 0.816 | UBQ2 | 1.183 | UBQ2 | 0.99 | |||
Best pair | UBQ1/ACT | 0.216 | ||||||||