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Table 3 Analyses of reference genes evaluated according to NormFinder and RefFinder algorithms

From: Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

Experimental condition NormFinder RefFinder
NormFinder geNorm BestKeeper DeltaCt
Gene Stability Gene Stability Gene Stability Gene Stability Gene Stability
Field UBQ1 0.271 UBQ1 0.365 ACT/RPL 0 GAPDH 0.539 UBQ1 0.770
ACT 0.350 25SrRNA2 0.494 UBQ2 0.559 ACT 0.591 25SrRNA2 0.820
25SrRNA2 0.351 TUB 0.620 UBQ1 0.713 RPL 0.591 RPL 0.860
RPL 0.358 UBQ2 0.629 GAPDH 0.759 UBQ1 0.617 ACT 0.860
UBQ2 0.367 GAPDH 0.640 TUB 0.769 25SrRNA2 0.630 GAPDH 0.880
TUB 0.404 RPL 0.663 25SrRNA2 0.800 TUB 0.639 TUB 0.880
GAPDH 0.418 ACT 0.663 25SrRNA1 0.892 UBQ2 0.663 UBQ2 0.880
25SrRNA1 0.525 25SrRNA1 1.054    25SrRNA1 0.930 25SrRNA1 1.170
Best pair UBQ1/ACT 0.164         
Greenhouse GAPDH 0.304 GAPDH 0.424 ACT/RPL 0 GAPDH 0.438 GAPDH 0.840
UBQ2 0.335 UBQ2 0.565 UBQ2 0.419 UBQ2 0.525 UBQ2 0.890
ACT 0.344 TUB 0.673 GAPDH 0.577 RPL 0.543 ACT 0.920
RPL 0.355 UBQ1 0.739 TUB 0.729 ACT 0.543 RPL 0.920
TUB 0.419 RPL 0.741 UBQ1 0.824 TUB 0.607 TUB 0.970
UBQ1 0.439 ACT 0.741 25SrRNA2 0.920 25SrRNA1 0.630 UBQ1 1.010
25SrRNA1 0.462 25SrRNA2 0.831 25SrRNA1 0.957 25SrRNA2 0.637 25SrRNA2 1.030
25SrRNA2 0.507 25SrRNA1 0.902    UBQ1 0.675 25SrRNA1 1.070
Best pair 25SrRNA1/UBQ2 0.211         
Hydropony—PEG8000 GAPDH 0.150 GAPDH 0.239 GAPDH/ACT 0.477 UBQ1 0.750 GAPDH 0.65
ACT 0.292 ACT 0.572 RPL 0.609 GAPDH 0.792 ACT 0.82
RPL 0.362 RPL 0.716 UBQ1 0.774 RPL 0.848 RPL 0.91
UBQ1 0.407 UBQ1 0.729 UBQ2 0.859 ACT 1.018 UBQ1 0.93
UBQ2 0.466 UBQ2 0.816    UBQ2 1.183 UBQ2 0.99
Best pair UBQ1/ACT 0.216