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Table 2 Composition of the 50 µL PCR reaction mixture used to amplify the 1812 bp insertional DNA fragment

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Component

Volume

dH2O

37.5 µL

10× polymerase buffer #3

5 µL

2.5 mM dNTP mix

4 µL

RIM-f2 (20 µM)

1 µL

RIM-r1 (20 µM)

1 µL

pSL72 plasmid template

1 µLa

Polymerase mix (5 U µL−1)

0.5 µL

  1. aContaining approx. 10 ng plasmid DNA
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Plant Methods

ISSN: 1746-4811