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Table 2 Composition of the 50 µL PCR reaction mixture used to amplify the 1812 bp insertional DNA fragment

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Component Volume
dH2O 37.5 µL
10× polymerase buffer #3 5 µL
2.5 mM dNTP mix 4 µL
RIM-f2 (20 µM) 1 µL
RIM-r1 (20 µM) 1 µL
pSL72 plasmid template 1 µLa
Polymerase mix (5 U µL−1) 0.5 µL
  1. aContaining approx. 10 ng plasmid DNA