|
Fluorophore
|
Calibration
|
Purge tubes of air using N2 gas or sodium dithionite
|
0.02–0.05
|
|
Sample preparation
|
Dissect tissue (e.g. scalpel, scissors or leaf punch) and place in measuring tube
|
0.5–1
|
|
Measurements
|
In general, slopes taken from 1 to 2.5-h. 186 samples per run. Note: more than 186 samples can be simultaneously measured but cycle time between O2 recordings will increase to >6-min, reducing resolution
|
0.8
|
|
Total
| |
1.3–1.9
|
|
O2-electrode
|
Calibration
|
Prepare and assemble electrodes, including application of membrane and electrode solution. Aerate calibration solutions and obtain zero and saturated O2 values after stabilisation of current
|
4–9
|
|
Sample preparation
|
Dissect tissue and place inside cuvette and adjust plunger being careful not to introduce air pockets
|
1–2
|
|
Measurements
|
Slopes taken after stabilisation of signal and before depletion of O2, usually within 10–40 min but dependent on sample
|
20–40
|
|
Total
| |
25–51
|
|
IRGA
|
Calibration
|
Change consumables (e.g. soda lime, desiccant, CO2 canister) and zero IRGA chambers
|
1–2
|
|
Sample preparation
|
Select and clip measuring chamber onto leaf
|
0.5–1
|
|
Measurements
|
Allow steady-state gas-exchange to be reached
|
10–15
|
|
Total
| |
11.5–18
|
|
MIMS
|
Calibration
|
Apply membrane and test membrane stability. Purge tube and inject known volumes of O2 and CO2. Record background consumption
|
5–10
|
|
Sample preparation
|
Dissect tissue and place inside cuvette and air-seal cuvette
|
1–3
|
|
Measurements
|
Allow signal to stabilise (usually 5 min) and record slope between 5 and 20 min
|
20
|
|
Total
| |
26–33
|
