Fig. 2
The influence of leaf tissue amount, CO2 concentrations, and mechanical wounding on the dark respiration rate (R dark) of wheat and Arabidopsis leaf tissue. a Differing amounts of leaf area in measuring tube volumes (cm2 mL−1) plotted against corresponding R dark for wheat (open blue circles) and Arabidopsis (open red squares). b R dark of wheat leaves sealed in measuring tubes with varying CO2 as a % of air. c The influence of mechanical wounding during sampling of leaf sections on the dark respiration rate (R dark) of two wheat cultivars, Janz and Ruby. The R dark of a 2 × 0.5 cm transverse section of leaf and a same sized leaf sliced a further 20 times was compared to an intact leaf, which was not mechanically damaged. Alternatively, leaves that were cut were washed with a wounding buffer prior to R dark measurements. The values are mean ± SE of four biological replicates. *significance at P < 0.05, ** at P < 0.01 and *** at P < 0.001, for a one-way ANOVA and a Dunnett’s multiple comparison test with the intact leaf set as the control