Table 2 Summary of methods used
From: Development of an efficient glucosinolate extraction method
Experiment | Fig | Species | Tissue | Freeze drying/tissue disruption | Extraction | Desulfation | HPLC |
|---|---|---|---|---|---|---|---|
1—Effect of freeze drier on GSL concentration | 2 | B. napus | Leaves | FD-A or FD-B/mill | Cold methanol | 0.3 U/ml for 24 h | ISO 9167-1 method |
2—Comparison of GSL extraction from freeze dried tissue with extraction from wet tissue | 3 | B. napus | Leaves | FD-A or −20 °C methanol | Cold methanol | 0.3 U/ml for 24 H | ISO 9167-1 method |
3—Comparison of extraction methods | 6, 7 | R. sativus B. juncea S. alba E. sativa | Leaves, stems, roots | FD-A | Hot methanol, Cold methanol, Boiling water | 0.3 U/ml for 24 H | ISO 9167-1 method |
4—Comparison of desulfation/purification methods | 8, 9 | R. sativus B. juncea S. alba E. sativa | Leaves, stems, roots | FD-A | Cold methanol | 0.3 U/ml for 12, 24, 48 h, and 5 U/ml for 16 h or filtration | ISO 9167-1 method for desulfoGSL, Herzallah and Holly method for intact GSLs |