Table 1 List of primer sequences used in this study
Primer | Sequence (5′–3′) |
|---|---|
VIGS construct | Â |
CS_lic_F | CGACGACAAGACCCTGAGAAGGGAAACTGCCATTTGA |
CS_An2_R | CTTTGTCTAGTG CCTTGCCCAGCTTTGTAATCTT |
GFP_lic_F | CGACGACAAGACCCTCACGGCAGACAAACAAAAGA |
GFP_An2_R | CTTTGTCTAGTG AAAGGGCAGATTGTGTGGAC |
An2_F | CACTAGACAAAGACGAACGCGAC |
An2_lic_R | GAGGAGAAGAGCCCTCAGAAAAGTCATCCCAACCATCAC |
PDS_lic_F | CGACGACAAGACCCTCTTGCAAAGATCCCCTGTAG |
PDS_An2_R | CTTTGTCTAGTGCACTTGTTTCTGCCAACTTC |
Sequencing | Â |
TRV2_seq_F | CTGTTTGAGGGAAAAGTAG |
TRV2_seq_R | CAAAAGACTTACCGATCAATC |
qRT-PCR | Â |
CS_F | TTCCCATATAGCCCACTTGCÂ |
CS_R | ACTACAAGCAAATTACCACCTTC |
PDS_F | AGCAAAGCAAAAATATTGAAGTA |
PDS_R | GCTTTCCTGATAAGACAGC |
An2_F | GGAGAAGGAAAGTGGCATCTTGT |
An2_R | CACCTCTCTTTATATGCGGCCTT |
CaActin_F | ATGGCAGATGAAGATATTCAAC |
CaActin_R | ACTAGGAAAAACAGCCCTTGGT |