|
VIGS construct
| |
|
CS_lic_F
|
CGACGACAAGACCCTGAGAAGGGAAACTGCCATTTGA
|
|
CS_An2_R
|
CTTTGTCTAGTG CCTTGCCCAGCTTTGTAATCTT
|
|
GFP_lic_F
|
CGACGACAAGACCCTCACGGCAGACAAACAAAAGA
|
|
GFP_An2_R
|
CTTTGTCTAGTG AAAGGGCAGATTGTGTGGAC
|
|
An2_F
|
CACTAGACAAAGACGAACGCGAC
|
|
An2_lic_R
|
GAGGAGAAGAGCCCTCAGAAAAGTCATCCCAACCATCAC
|
|
PDS_lic_F
|
CGACGACAAGACCCTCTTGCAAAGATCCCCTGTAG
|
|
PDS_An2_R
|
CTTTGTCTAGTGCACTTGTTTCTGCCAACTTC
|
|
Sequencing
| |
|
TRV2_seq_F
|
CTGTTTGAGGGAAAAGTAG
|
|
TRV2_seq_R
|
CAAAAGACTTACCGATCAATC
|
|
qRT-PCR
| |
|
CS_F
|
TTCCCATATAGCCCACTTGC
|
|
CS_R
|
ACTACAAGCAAATTACCACCTTC
|
|
PDS_F
|
AGCAAAGCAAAAATATTGAAGTA
|
|
PDS_R
|
GCTTTCCTGATAAGACAGC
|
|
An2_F
|
GGAGAAGGAAAGTGGCATCTTGT
|
|
An2_R
|
CACCTCTCTTTATATGCGGCCTT
|
|
CaActin_F
|
ATGGCAGATGAAGATATTCAAC
|
|
CaActin_R
|
ACTAGGAAAAACAGCCCTTGGT
|
