Fig. 3

Application of the FDIT method in a kinase reaction. a Effect of reaction time. ATP was added to initiate auto-phosphorylation (WKS1.1) or the kinase reaction (WKS1.1 + tAPX) of WKS1.1 and the reactions were stopped at different times. Samples without ATP serve as negative controls. b Effect of the kinase amount. ***Student’s t test p value <0.001, **student’s t test p value <0.01, *student’s t test p value <0.05. n = 4. Bars stand for ± standard error