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Fig. 1 | Plant Methods

Fig. 1

From: Infiltration-RNAseq: transcriptome profiling of Agrobacterium-mediated infiltration of transcription factors to discover gene function and expression networks in plants

Fig. 1

Agroinfiltration of 35S:MtLAP1 induces anthocyanin pigment production in Medicago truncatula leaves. a Experimental design of M. truncatula MtLAP1 Infiltration-RNAseq experiment. On Day 0 (top) all three leaflets of the 4th trifoliate leaf of healthy 3 weeks old plants were agroinfiltrated (represented by syringe) with ‘Control’ infiltrations (left hand side) or ‘LAP1’ infiltrations (right hand side). For the Control infiltrations, a single plant was infiltrated with either: 35S:MtCOla, 35S:MtCOlf or 35S:MtFTa1plusMtFD [56, 57]. For the LAP1 infiltrations, a triplicate set of plants were infiltrated with 35S: MtLAP1 [25]. Four days post infiltration (bottom), all three leaflets of agroinfiltrated leaves were harvested (represented by scissors) in preparation for RNA extraction and analysis. The resulting control samples were: 35S:MtCOla, 35S: MtCOlf and MtFTa1plusMtFD, and the resulting LAP1 samples (purple leaves due to anthocyanin production) were 35S: MtLAP1_1, 35S: MtLAP1_2 and 35S: MtLAP1_3. b All three leaflets of the 4th trifoliate leaf of healthy 3 weeks old plants were agroinfiltrated with 35S:MtLAP1, 35S:MtCOla or 35S:MtCOlf. The 35S:MtCOla and 35S:MtCOlf constructs overexpress Medicago genes that are not involved in anthocyanin biosynthesis [56, 57] (see “Methods”). Agroinfiltrated leaves were harvested for photographing 4 days post infiltration. Note: these leaves are representative agroinfiltrations and were not used for RNA extraction and downstream analyses

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Plant Methods

ISSN: 1746-4811