Fig. 5From: An automated, cost-effective and scalable, flood-and-drain based root phenotyping system for cerealsSteps of the non-invasive imaging of root systems followed by the process of image analysis. a Root staining with powdered active charcoal. b Root imaging using a RGB camera. Pictures are taken at different positions of the rotated acrylic tube with shifts of 45°. c The process of merging the panorama picture from the single pictures using a Panorama Maker 6 (ArcSoft) software. d Analysis of the root picture using a NeuronJ plugin to ImageJ software. The tracked primary roots are marked red whereas laterals are marked pink. e Result page of the analysis carried out using a NeuronJ plugin. Result table contains the information on assigned labels and measurements (lengths in pixels)Back to article page