Skip to main content
Fig. 4 | Plant Methods

Fig. 4

From: Efficient multiplex mutagenesis by RNA-guided Cas9 and its use in the characterization of regulatory elements in the AGAMOUS gene

Fig. 4

Functional analysis of a regulatory region in the second intron of AGAMOUS. a Binding profiles of MADS-domain transcription factors (TFs) in the second intron of AGAMOUS. Cross symbols indicate the expected cutting regions. b Ten individuals from the progeny of a T1 plant were genotyped for presence of mutation (①) and T-DNA (②). The mutation was confirmed by sequencing (③). Red boxes in ③ indicate PAM motifs. c Homozygous mutant shows partial homeotic transformations of stamens to petals (II–V, I is wild type flower)

Back to article page

Plant Methods

ISSN: 1746-4811