Fig. 2
From: The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat
Bivariate flow karyotypes of three transgenic HOSUT lines of wheat obtained after the analysis of chromosomes with FITC-labelled (GAA)n microsatellites and stained by DAPI. The position of red and green regions used to sort particular chromosomes is indicated. The green sort gate was found to represent chromosomes carrying transgene. Chromosomes were flow-sorted also from the neighboring population delineated by red gate and were used as a control. Although the transgene-bearing chromosome should not be included in this region, the sorted population could potentially be contaminated with transgene-bearing chromosomes due to similarity in chromosome size and DNA content