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Fig. 1 | Plant Methods

Fig. 1

From: Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

Fig. 1

Grafting procedure of B. napus seedlings. 3 day old B. napus seedlings were first transferred to a cutting plate covered with moistened filter paper (a). After removing of the cotyledons with a razor blade (b), 1–2 cm of the middle of the hypocotyls where removed (c, d). Finally the cut plant parts [shoot (s1 and s2) and root (r1 and r2)] were transported to a regeneration plate (e) and joined using tweezers (f). Therefore the shoot of the one seedling (s1) were connected to the root of the other plant (r2)

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Plant Methods

ISSN: 1746-4811