Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce

Table 1 Candidate reference genes selected for testing

Gene symbol	Definition	Arabidopsis thaliana homolog locus tag	References	Lettuce Genbank ID
UBQ1	Putative ubiquitin extension protein	AT3G52590.1	[41]	NM_129273.4
UBQ7	Ubiquitin-like protein RUB2	AT2G35635	[36]	NM_129118
ACT2	Actin 2	At3g18780	[5]	AK317453.1
ACT12	Actin protein coding 12	At3G46520	[37]	BT005073.1
TUA-3	Tubulin alpha-3	At5g19770	[38]	BT000718.1
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase, cytosolic	At1g13440	[5, 37, 40]	AK317337.1
UBC9	Ubiquitin conjugating enzyme 9	At4g34270	[39]	AF325019.2
TIP41	TIP41-like protein	AT4G34270	[5, 11, 39, 42]	NM_119592.4
PP2AA3	Protein phosphatase 2A regulatory subunit A3	At1g13320	[5, 39]	BT002601.1
PP2A-1	Protein phosphatase 2A-1	At1g59830	[5]	AY096543.1

ISSN: 1746-4811