Fig. 3

Effects of MG132 on levels of newly synthesized proteins in roots using SUnSET. Plants were treated with or without 50 μM MG132 and subsequently treated with or without 20 μM puromycin. Twenty μg of root protein were separated on a 15 % SDS-PAGE gel, and newly synthesized proteins were detected using puromycin antiserum. Membranes were stained with Ponceau Red prior to immunblotting. Smaller letters in parenthesis represent band intensity relative to control on dried membranes using image-J. Images are representative of four experimental replicates. Levels of Bip2 were analyzed from the same membrane. kDa—kilodalton