Table 1 List of TALEN and RGEN target site sequences and experiments used
From: A simple test for the cleavage activity of customized endonucleases in plants
Construct | Target sequence | Species | Approach (constructs) |
|---|---|---|---|
pTARGET-gfp1 | TGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAAGT | Tobacco | RGENt, st (pSI24) |
TALENt, st (pSP10, pSP11) | |||
Barley | TALENt, st (pGH297, pGH400) | ||
pTARGET-gfp2 | GTCTTTGCTCAGGGCGGACTGGG | Barley | RGENt (pSH92) |
MLO pair #1 | TGGTGCTCGTGTCCGTCCTCATGGAACACGGCCTCCACAAGCTCGGCCATGTA | Tobacco/barley | TALENt (p110/111) |
MLO pair #2 | TCCTCATGGAACACGGCCTCCACAAGCTCGGCCATGTAAGTCCCGTTACCCTA | TALENt (p112/113) | |
MLO pair #3 | TGGAACACGGCCTCCACAAGCTCGGCCATGTAAGTCCCGTTACCCTAGCTCAA | TALENt (p114/115) | |
MLO pair #4 | TGCTGGCTTTGTATGCAGATGGGATCAAACATGAAGAGGTCCATCTTCGACGA | TALENt (p124/125) | |
MLO pair #5 | TGGCTTTGTATGCAGATGGGATCAAACATGAAGAGGTCCATCTTCGACGAGCA | TALENt (p126/127) | |
pTARGET-MLO | GCTGGAACACGGCCTCCACAAGCTCGGCCATGTAAGTCCCGTTACCCTAGCTCA | Barley | TALENt, st (p114/115) |