Fig. 3

Alignment of mutated gfp sequences recovered from four barley transformants (BM33_639, BM35_697, BM36_757, and BM37_760) induced by the presence of a TALEN pair. gfp-specific PCR products were subcloned and up to ten clones were sequenced. The sequences shown in red and blue represent, respectively the left and right TALEN binding sites; the number of nucleotides deleted (dashes) and the number of mutants/number of clones analysed is shown to the right of each sequence