Fig. 6

Simultaneous RNAi mediated down-regulation of expression of seven target genes in Arabidopsis hpRNAi lines transformed with a single RNAi construct. a Phenotype of one T1 individual of Arabidopsis transformed with pAGRIKOLA-LNGTMHS. b Leaves of one T1 individual of Arabidopsis transformed with pAGRIKOLA-LNGTMHS, and one T1 individual transformed with pAGRIKOLA-NLSHMTG were tested for expression of each of the target genes, AtGUN4, AtHY2, AtTRY, AtLNG1, AtNPQ1, AtSEX1 and AtMAX3. Abundance of the respective target transcripts are given as relative expression \((2^{{ - \Delta \Delta {\text{C}}_{\text{T}} }} )\) compared to that of an Arabidopsis line expressing a pAGRIKOLA-Control RNAi fragment and AtACT2 for normalization