Fig. 4

Elicitor dose dependency in the monocot Oryza sativa. Oxidative burst of Oryza sativa cell suspension cultures elicited with different concentrations of chitosan (0.01–0.33 mg/ml) in triplicates. During the 120-min measuring period, a first peak with high amplitude and short duration was observed followed by a second peak with longer duration (a). Each peak maximum was used to plot dose-dependency curves, in which peak 1 is defined as 0–20 min and peak 2 as 25–100 min (b). The data shown in a are taken from one experiment which is representative of ten independent experiments. The data shown in b are mean values ± SD of these ten experiments with the maximum relative light units (RLUs) reached in each experiment set to 100 %