Fig. 2

Elicitor dose dependency in the dicot Medicago truncatula. Dose dependency of the oxidative burst in Medicago truncatula cell suspension cultures elicited with different concentrations of ulvan (0.05–0.46 mg/ml) in triplicates. During the 90-min measuring period, first a peak with high amplitude and short duration was observed followed by a second peak with lower amplitude and longer duration (a). Each peak maximum was used to plot dose-dependency curves, in which peak 1 is defined as 0–20 min and peak 2 as 25–60 min (b). The data shown in a are taken from one experiment which is representative of six independent experiments. The data shown in b are mean values ± SD of these six experiments with the maximum relative light units (RLUs) reached in each experiment set to 100 %. P values resulting from a Whitman-rank test were combined and showed that the two curves were significantly different