Fig. 1From: Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/CasSchematic representation of the Cas9 cleavage mechanism. The Cas9/sgRNA complex recognizes and binds the complementary sequence next to the PAM, which is highly specific for each Cas9 from different bacterial species. The RuvC domain and HNH motif of Cas9 cleave the two DNA strands 3 bp upstream of the PAMBack to article page