Fig. 5
From: Optimisation approaches for concurrent transmitted light imaging during confocal microscopy
Water infiltration through de-gassing improved transmitted light imaging of leaves. Images show the epidermis and palisade mesophyll of a young Arabidopsis leaf, and are fluorescence with an overlay of YFP-HDEL (green) and chlorophyll autofluorescence (red) and 561 nm green transmitted light images. a Control. b Leaf de-gassed (2 × 5 min)—the fluorescence images were unaffected by the de-gassing treatment which removed air pockets behind the image plane. Scale bar 50 µm