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Fig. 5 | Plant Methods

Fig. 5

From: A high-throughput pipeline for detecting locus-specific polymorphism in hexaploid wheat (Triticum aestivum L.)

Fig. 5

The pipeline of generating multiple sequence alignments in bread wheat. CSSs chromosome shotgun sequences; A, B, and D the three subgenomes of bread wheat; C1, C2, and C3 the three contigs from the three subgenomes; ‘CS’ euploid wheat ‘Chinese Spring’; NATB, NBTA, and NDTB 3 nulli-tetrasomic (NT) lines for chromosomes belonging to a given homoeologous group. Asterisks representing polymorphic bases targeted in designing primers specific to a subgenome. The lack of a PCR product in the lane of nulli-A and tetra-B (or NATB) indicates that primers are specific to chromosome A.

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Plant Methods

ISSN: 1746-4811