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Figure 3 | Plant Methods

Figure 3

From: A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages

Figure 3

Use of Arabidopsis pin-fasten grafting for hypocotyl grafting. Two-month-old SD-grown Arabidopsis wild-type (Col) and gl1-1 plants were used as stocks (a) and scions (b), respectively. c Two to 3 leaves near the hypocotyl of stock were removed to expose the hypocotyl. d The epidermis of the hypocotyl was removed by use of blades. The scraped epidermis is indicated by an arrow. Scale bar is 1 mm. e gl1-1 scions (right) were pin-fastened on stock hypocotyls (left). The insect pins are indicated by red arrowheads. f, g Images of successful grafts at 2 weeks after grafting. The newly developed glabrous leaves of scions are indicated by white asterisks in (g).

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