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Table 3 Detection success of amplicons yielded from species mixtures containing identified and undetermined plant species

From: Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots

cpDNA Fragment size (bp) Species Soil sourcea Detection rate
4.5 ng μL−1 DNA 25 ng μL−1 DNA 45.5 ng μL−1 DNA
trnT-trnL intergenic spacer 531 Undetermined 1, 2 0/6 1/6 5/6
trnT-trnL intergenic spacer 637 Undetermined 1 0/3 2/3 3/3
trnT-trnL intergenic spacer 679 Undetermined 2 0/3 6/6 3/3
trnT-trnL intergenic spacer 881 Undetermined 1, 2 0/6 6/6 6/6
trnT-trnL intergenic spacer 1,154 Melilotus officinalis n/a 0/6 6/6 6/6
cpDNA Fragment size (bp) Species Soil sourcea Detection rate
0.09 ngμL−1 DNA 0.5 ng μL−1 DNA 0.9 ng μL−1 DNA
trnL intron 320 Undetermined 1 0/3 2/2 3/3
trnL intron 490 Undetermined 1 3/3 2/2 3/3
trnL intron 504 Undetermined 3 3/3 0/3 3/3
trnL intron 508 Undetermined 1 3/3 2/2 3/3
trnL intron 512 Undetermined 3 3/3 3/3 3/3
trnL intron 522 Undetermined 1 3/3 2/2 3/3
trnL intron 560 Undetermined 1, 3 6/6 5/5 6/6
trnL intron 584 Undetermined 3 0/3 0/3 3/3
trnL intron 604 Undetermined 3 3/3 3/3 3/3
trnL intron 696 Populus tremuloides n/a 0/6 5/5 6/6
trnL-trnF intergenic spacer 179 Undetermined 5 3/3 3/3 3/3
trnL-trnF intergenic spacer 300 Undetermined 4 3/3 3/3 3/3
trnL-trnF intergenic spacer 324 Undetermined 4 3/3 3/3 3/3
trnL-trnF intergenic spacer 387 Undetermined 4, 5 6/6 6/6 6/6
trnL-trnF intergenic spacer 391 Undetermined 4 3/3 3/3 3/3
trnL-trnF intergenic spacer 394 Bromus inermis b n/a 3/3 6/6 9/9
trnL-trnF intergenic spacer 402 Undetermined 4 3/3 3/3 3/3
trnL-trnF intergenic spacer 432 Undetermined 4 3/3 3/3 3/3
trnL-trnF intergenic spacer 443 Bromus inermis b n/a 3/3 6/6 9/9
trnL-trnF intergenic spacer 460 Undetermined 4, 5 6/6 3/3 6/6
  1. DNA was extracted from leaves of an identified species and mixed in varied proportions with DNA extracted from unidentified roots in soil. Numerator is number of successful detections; denominator is number of trials. See text for description of soil source.
  2. aRoots from individual soil samples yielded fragments of different sizes; the genomic material yielding these fragments occurred in an unknown proportion, which comprised the DNA template.
  3. bThis species yielded fragments of different sizes.