TRiP: Tracking Rhythms in Plants, an automated leaf movement analysis program for circadian period estimation

Table 1 Period estimation using TRiP to analyze simulated data with different amplitude trends and noise levels

Known	Amplitude ²	Noise level A ³	Noise level B ³	Noise level C ³
Period ¹		Period	Period	Period
(h)		(h; mean ± sd)	(h; mean ± sd)	(h; mean ± sd)
20	I	20.00 ± 0.02	19.98 ± 0.06	19.99 ± 0.12
21	I	21.04 ± 0.07	21.07 ± 0.09	21.07 ± 0.12
22	I	22.08 ± 0.07	22.11 ± 0.09	22.10 ± 0.18
23	I	23.08 ± 0.07	23.09 ± 0.07	23.07 ± 0.18
24	I	23.99 ± 0.02	24.00 ± 0.04	24.06 ± 0.15
25	I	25.05 ± 0.06	25.05 ± 0.11	25.05 ± 0.26
26	I	26.09 ± 0.14	26.08 ± 0.24	25.98 ± 0.26
27	I	27.11 ± 0.12	27.20 ± 0.12	27.10 ± 0.34
28	I	28.13 ± 0.16	28.12 ± 0.22	28.14 ± 0.27
20	II	20.00 ± 0.03	20.00 ± 0.12	19.97 ± 0.10
21	II	21.06 ± 0.06	21.05 ± 0.09	21.06 ± 0.21
22	II	22.08 ± 0.09	22.10 ± 0.07	22.04 ± 0.14
23	II	23.08 ± 0.07	23.12 ± 0.12	23.05 ± 0.22
24	II	24.00 ± 0.04	23.98 ± 0.09	24.06 ± 0.19
25	II	25.07 ± 0.06	25.06 ± 0.13	25.07 ± 0.28
26	II	26.15 ± 0.11	26.07 ± 0.09	26.08 ± 0.27
27	II	27.16 ± 0.14	27.14 ± 0.14	26.99 ± 0.31
28	II	28.17 ± 0.15	28.19 ± 0.20	28.17 ± 0.31
20	III	20.00 ± 0.03	20.00 ± 0.07	20.06 ± 0.15
21	III	21.07 ± 0.05	21.08 ± 0.14	21.12 ± 0.20
22	III	22.09 ± 0.09	22.12 ± 0.09	22.02 ± 0.16
23	III	23.08 ± 0.08	23.04 ± 0.12	23.04 ± 0.18
24	III	24.00 ± 0.02	23.96 ± 0.12	24.08 ± 0.20
25	III	25.07 ± 0.05	25.08 ± 0.15	24.97 ± 0.19
26	III	26.18 ± 0.11	26.12 ± 0.19	26.22 ± 0.29
27	III	27.15 ± 0.14	27.18 ± 0.14	27.01 ± 0.17
28	III	28.18 ± 0.12	28.17 ± 0.17	28.16 ± 0.38

¹A cosine of known frequency.
²Three levels of amplitude trends, defined as the rate at which the amplitude envelope of the signal decays, were applied: I = 0, II = 0.001, III = 0.002.
³Noise levels (A =0.2 amplitude, B =0.6 amplitude, C =1 amplitude).
The mean and standard deviation were calculated from 10 repeated simulations. Circadian periods plotted in Figure 2 were calculated using TRiP.

ISSN: 1746-4811