Figure 1

Work flow of the R script. The users upload the previously captured fluorescence images and/or their associated histograms as input. The data are sorted out according to the information indicated by the user on the web interface. To get an overview of the data, the data are represented in the form of contact sheets of images or of histograms (A). Here, A. thaliana plants were imaged 15, 17 and 21 days after inoculation (d.a.i.) by P. ramosa. Images and histograms are displayed on the contact sheet to visualize a variation during time. The mean of the global CF values for each treatment are graphically represented in a radial plot where each point represents the mean for one treatment and each radius represents a CF parameter (B). In order to detect a significant difference in the global CF values between the treatments, the means of the global CF values for each treatment are represented in the form of a box plot (C) in which horizontal lines indicate the 0.25 and 0.75 percentile from bottom to top, the interior line indicates the median and the exterior shapes represent the overall distribution. The results of the Mann–Whitney U tests are indicated in box plots. To compare the various histograms, a dendrogram based on the histograms is produced (D).