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Table 3 Oligonucleotides used

From: TETX: a novel nuclear selection marker for Chlamydomonas reinhardtii transformation

Name

Sequence 5′-3′

aph8F

CGTGCACTGCGGGGTCGGT

aph8R

CCGCCCCATCCCACCCGC

ble1F

CCGGGTCGCGCAGGGC

ble1R

GCGCCGTTCCGGTGCTCA

tetXhF

TCTCGAGATGACCATGCGCATCGACACCGA

tetBaR

TGGATCCTCACACGTTCAGCAGCAGCTGCTG

  1. Listing of oligonucleotides used in this work. Bases in bold indicate recognition sites for restrictions enzymes and accessory bases used for cloning purposes.
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Plant Methods

ISSN: 1746-4811