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Table 3 SNVs found in four PME genes

From: Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome

Gene

Base No.

Change

Sanger confirmation

Nucleotide substitution

Amino acid substitution

LuPME79

33

G > A

No

N/A

N/A

LuPME79

96

G > A

Yes

Heterozygous

Non-synonymous

LuPME73

25

G > A

No

N/A

N/A

LuPME73

54

G > A

Yes

Heterozygous

Non-synonymous

LuPME73

81

T > A*

No

N/A

N/A

LuPME73

88

C > T

Yes

Heterozygous

Synonymous

LuPME73

97

A > G*

No

N/A

N/A

LuPME73

139

C > T

Yes

Homozygous

Synonymous

LuPME73

189

C > T

No

N/A

N/A

LuPME10

154

C > T

Yes

Homozygous

Synonymous

LuPME105

34

A > G*

N/A

N/A

N/A

LuPME105

57

A > G*

No

N/A

N/A

LuPME105

115

G > A

No

N/A

N/A

  1. N/A – sequence could not be obtained by Sanger.
  2. *Mutations not expected by EMS, but discovered using the technique.
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Plant Methods

ISSN: 1746-4811