Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome

Table 2 Read statistics of the three experiments performed

Experiment/replicate	Scaffold ID/gene ID	Gene name/annotation ¹	Number of reads mapped in all 28 pools	Percentage of mapped reads in all 28 pools ²	Average read coverage per pool
Pilot	S20	N/A	115,998	17.09	4,103.69
	S411	N/A	67,971	10.02	2,392.08
	S900	N/A	137,314	20.23	4,794.04
Proof of concept-1	Lus10004720	LuPME10	91,755	19.95	2,975.50
	G25305^*	LuPME73	94,242	20.49	2,942.75
	Lus10031470	LuPME79	38,154	8.30	1,059.81
	Lus10043035	LuPME105	53,216	11.57	1,621.20
Proof of concept-2	Lus10004720	LuPME10	87,889	16.17	2,815.02
	G25305^*	LuPME73	143,832	26.46	4,404.28
	Lus10031470	LuPME79	47,620	8.76	1,255.62
	Lus10043035	LuPME105	63,282	11.64	1,941.29
Scale up	Lus10016751	ALS-1	1,443,997	42.43	44,199.34
	Lus10029955	ALS-2	424,425	12.47	13,027.62
	G24175^*	CLE	534,255	15.70	16,146.73
	Lus10017825	UGT	729,799	21.44	20,472.63

^*Gene Id correspond to first draft assembly of flax (unpublished).
¹PME = Pectinmethylesterase, ALS = acetolactate synthase, CLE = cyclic peptide, UGT = glucoronosyl/glucosyl transferase.
²Percentage from total number of reads in Table 1.

ISSN: 1746-4811