Table 1 DNA sequence parameters of optimized plant transporter genes OsNAR2.1 and OsNRT2.3a
From: Optimizing plant transporter expression in Xenopus oocytes
OsNAR2.1 | OsNRT2.3a | |||||
|---|---|---|---|---|---|---|
Synthetic | Original | Synthetic | Original | |||
GC content (%) | 52.6 | 71.0 | 49.0 | 67.2 | ||
Repeat sequence | Max direct repeat | Size: | 8 | 11 | 10 | 13 |
Distance: | 7 | 177 | 984 | 993 | ||
Frequency: | 2 | 2 | 2 | 2 | ||
Max inverted repeat | Size: | 10 | 11 | 12 | 11 | |
Tm | 27.3 | 55.7 | 48.8 | 53.6 | ||
Start positions | 3, 475 | 165, 54 | 230, 1497 | 764, 615 | ||
Max dyad repeat | size | 9 | 11 | 10 | 11 | |
Tm | 27.5 | 59.8 | 31.9 | 50.2 | ||
Start positions | 151, 450 | 363, 540, 73 | 198, 1156 | 309, 1442 | ||
Cis-acting element | PolyT | 0 | 0 | 0 | 0 | |
PolyA | 0 | 0 | 0 | 0 | ||