Schematic representation of the principal steps performed in the protocol for immunodetection of histone methylation. Different tissues were collected and fixed with FAA solution and paraffin-embedded. The samples were sectioned into 4-5-μm slices and the slides were dewaxed. The antigenic sites were retrieved and the tissue was blocked and incubated with the primary and secondary antibodies. The samples were counterstained with DAPI and analyzed under confocal microscope.