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Table 3 Summary of SSR primers tested, quality of PCR products and polymorphisms detected

From: Validation of doubled haploid plants by enzymatic mismatch cleavage

Primer pair1

PCR amplification2

Polymorphisms detected between parents

 

GP

HOR1606

GP + HOR1606

cnl34

W

W

N

cnl73

?

?

N

cnl31

Y

Y

N

cnl146

Y

Y

Y

cnl130

Y

Y

?

cnl151

N

Y

---

cnl140

N

N

---

HVACL1

Y

Y

?

HVLEU

Y

Y

N

HVGNIRE

Y

Y

N

HVCSG

N

N

---

HVADH1

N

N

---

HVWAXYG

?

?

N

HVDHN7

Y

?

?

HVCMA

Y

Y

Y

HVDHN9

Y

Y

N

HVBKASI

Y

W

?

HVPRP1B

?

Y

?

HVBDG

W

W

N

HVBARE1

N

N

---

HVRCABG

W

N

---

HVSIP1A

Y

Y

N

Bmac0113

Y

Y

Y

Bmag0323

Y

Y

N

Bmac0812

Y

Y

N

Bmag0477

W

W

?

Bmag0387

Y

W

?

Bmac0163

Y

Y

N

GBM1284

?

?

---

Bmag0346

N

N

---

Bmag0023

N

N

---

GBM1300

Y

Y

N

  1. 1From http://germinate.scri.ac.uk/ssr/barley_s.html.
  2. 2Y = yes, W = weak amplification, N = no, ? = unclear results, --- = not tested.
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Plant Methods

ISSN: 1746-4811