Table 1 Primer sequences, PCR product sizes, and references for enzymatic mismatch cleavage
From: Validation of doubled haploid plants by enzymatic mismatch cleavage
Number | Primer name | Primer sequence | Product size (bp) | Source |
|---|---|---|---|---|
1 | rdg2a_F1 | CTTGCTCTCAAGACAATGGGTGGATTG | 1499 | 1 |
| Â | rdg2a_R1 | TCCAAACTGCTAAACATCCGAGGCTCT | Â | Â |
2 | rdg2a_F2 | CTCTCAAGACAATGGGTGGATTGCTGA | 1493 | 1 |
| Â | rdg2a_R2 | CAAACTGCTAAACATCCGAGGCTCTCC | Â | Â |
3 | rdg2a_F3 | TCGCTATGTCAAGAGCTGGATGAAGGA | 1496 | 1 |
| Â | rdg2a_R3 | AAGTGCGTAGGATTGTTCTGCCTTTGC | Â | Â |
4 | nbs2-rdg2a_F1 | GCTCTTCCGTTTTGAAATGAGCAGGAA | 1503 | 1 |
| Â | nbs2-rdg2a_R1 | TGTTTTGCATTTATGGCCTTGCAAATG | Â | Â |
5 | nbs2-rdg2a_F2 | TCCACTACCCGAAAGGCACTCAGCTAC | 1500 | 1 |
| Â | nb2-rdg2a_R2 | GCAATGCAATGCTCTTACTGACGCAAA | Â | Â |
6 | nb2-rdg2a_F3 | TCGAACGAATCAGTGGGTTATGCAAAG | 1497 | 1 |
| Â | nb2-rdg2a_R3 | ATGAAGTGTTCCCCTCCAGGTTGTCAC | Â | Â |
7 | nbs3-rdg2a_F1 | TGGCAAGTCCACTACCAAAAAGGCACT | 1491 | 1 |
| Â | nbs3-rdg2a_R1 | GCTCTTAGTGATGCCAATACCCGTTGC | Â | Â |
8 | nbs3-rdg2a_F2 | GCTCTTCCGTTTTGAAATGAGCAGGAA | 1491 | 1 |
| Â | nbs3-rdg2a_R2 | TGTTTTGCATTTATGGCCTTGCAAATG | Â | Â |
9 | HVgna1f | GACCCAGATGGCATCCAC | 552 | 2 |
| Â | HVgna1r | ATGCGACGAGACAAAGGAAT | Â | Â |
10 | HVraa1f | GTCGACGACTTGCATCATCTATCG | 545 | 2 |
| Â | HVraa1R | CACCCCGATCACTAACACACAA | Â | Â |
11 | HV_hpa1F | CCCTTATGTGTACCCTGATCCTGA | 1100 | 2 |
| Â | HV_hpa1R | GGTCCAACAGACGTATTAGCCAAG | Â | Â |
12 | HV_Mlo9-F1 | AGCAAACCAGACACACAGCAGCGTACC | 900 | 3 |
| Â | HV_Mlo9-R1 | GCAAAGGCTCACTTTGAGACGGCTTAG | Â | Â |
13 | HV_Mlo9-F2 | CATTTGTCGCAAAACAGCAAGTTCGAC | 1476 | 3 |
| Â | HV_Mlo9-R2 | TTGTCTCATCCCTGGCTGAAGGAAAAA | Â | Â |
14 | HvHox1-F1 | AAGCATGGACAAGCATCAGCTCTTTGA | 985 | 3 |
| Â | HvHox1-R1 | GGCAGCAGCTATCTCGGCTATTTTATGG | Â | Â |
15 | HV_Kap1_c1_F | TCCACCGGTAAAGAAACCAG | 1030 | 4 |
| Â | HV_Kap1_c1_R | TGAGGGAGGGAGAAAGATGA | Â | |
16 | HV_Kap1_d1_F | CTCCCTCCCTCAAGAAATCC | 899 | 4 |
| Â | HV_Kap1_d1_R | GCTGTCGCAAAATACAGCAA | Â | |
17 | HV_Kap1_e1_F | TTGCTGTATTTTGCGACAGC | 1136 | 4 |
| Â | HV_Kap1_e1_R | CATGTGTTAAAAGCCGCAGA | Â | |
18 | HV_Kap1_g1_F | ATTGAGTGCCTCTCGGCTTA | 1175 | 4 |
| Â | HV_Kap1_g1_R | TGAGGAAAGAAGGGATGTGG | Â | |
19 | HV_Kap1_h1_F | CCACATCCCTTCTTTCCTCA | 718 | 4 |
| Â | HV_Kap1_h1_R | GGGAGCTTGCCTTTCTTCTT | Â | |
20 | HV_Kap1_i1_F | TGTGGAACTATAAATCTGGCTTCA | 698 | 4 |
| Â | HV_Kap1_i1_R | CGAGCTAGCCGAACCTGTAG | Â | |
21 | Hv_mloA-F | CGTGTGCGTACCTGGTAGAG | 599 | 5 |
| Â | Hv_mloA-R | CAAGCCAAGACGACAATCAG | Â | |
22 | Hv_mloB-F | CTGATTGTCGTCTTGGCTTG | 624 | 5 |
| Â | Hv_mloB-R | CTGACTCCATACGCCAAACA | Â | |
23 | Hv_mloC-F | TGTTTGGCGTATGGAGTCAG | 566 | 5 |
| Â | Hv_mloC-R | AGAAACCGGAGAGGAGAAGG | Â | |
24 | Hv_mloD-F | CCTCACCCTCTTCCTTGACA | 574 | 5 |
| Â | Hv_mloD-R | CGTCAGAGCAGTTCATCAGC | Â | |
25 | Hv_mloE-F | CCACCGATGAACTTGTCAGT | 837 | 5 |
| Â | Hv_mloE-R | GAGAGGGGTTTTGTTTGTGC | Â | |
26 | Hv_Mla1-3Â F | AGCAGCTCGACAGCCAAGACAA | 451 | 5 |
| Â | Hv_Mla1-3R | CCCAACCCTCCAAATCCAACAA | Â | Â |