Gas exchange measurements for Arabidopsis Col-0 measured using the LiCOR extended reach chamber or whole plant chamber whilst growing in hydroponics. A Transpiration or B Net CO2 assimilation/photosynthesis measured using 6-week old plants growing the basal nutrient solution. Individual plants were exposed to light intensity of ~350 μmol m-2 s-1 at least 30 min prior to the start of measurement. The rosette was allowed to acclimatise inside the Arabidopsis whole rosette or extended reach chamber for at least 10 min before gas exchange data were recorded with reference CO2 concentration set at 500 μmol mol-1, flow rate at 500 μmol s-1 (for the whole plant chamber) or 100 μmol s-1 (for the extended reach chamber) light intensity at 350 μmol photons m-2 s-1 and relative humidity at 56%. Data shown as Mean + SEM of fifteen biological replicates. No significant differences were found between each dataset using a t-test (P < 0.01).