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Figure 3 | Plant Methods

Figure 3

From: Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families

Figure 3

Preparations of nuclei obtained following the final purification on 35% Percoll cushion by centrifugation. Preparations of nuclei from leaves of (A) tobacco, (B) potato and (C) apple obtained after the final purification on 35% Percoll cushion by centrifugation (step 6 in Figure 1A) contained only negligible amounts of debris. DAPI-stained nuclei (shown in blue) were observed with a fluorescence microscope using UV-light and debris observed simultaneously under normal light. The unstained structures are remaining contaminants. Scale bars, 10 μm.

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Plant Methods

ISSN: 1746-4811