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Figure 1 | Plant Methods

Figure 1

From: A fluorometric assay for trehalose in the picomole range

Figure 1

Principle of the fluorometric assay of trehalose. (A) First, glucose is eliminated from the tissue extract by incubation with glucose oxidase and catalase. (B) After inactivation of the glucose oxidase and catalase, trehalose is then hydrolysed to glucose by trehalase. Glucose is determined by coupling to peroxidation of a fluorogenic substrate, Amplex Red®, using glucose oxidase and peroxidase and measuring the increase in fluorescence at 590 nm.

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