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Figure 6 | Plant Methods

Figure 6

From: Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples

Figure 6

The elution profiles of pigments from extracts separated by HPLC. Representative HPLC profiles from each experiment are shown. A. HPLC profile of pigments extracted by immersing wild-type (WT) leaves in pure acetone for 12 hours at 4°C. B. An HPLC profile of WT pigments extracted by the quick boiling method. C. HPLC profile of pigments extracted from leaves of WT plants by immersing leaves in pure acetone for 96 hours at −30°C. D. HPLC profile of pigments extracted from leaves of WT plants by immersing leaves in ethanol at 4°C for 12 hours. E. HPLC profile of pigments extracted from leaves of WT plants by immersing leaves in DMF at 4°C for 12 hours. Peak 1, chlorophyllide b. Peak 2, chlorophyllide a, Peak 3, pheophorbide a. Peak 4, neoxanthin. Peak 5, violaxanthin. Peak 6, lutein. Peak 7, chlorophyll b. Peak 8, chlorophyll a. Peak 9, pheophytin a. Peak 10, β-carotene.

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