Emission spectra profiling of fluorescent proteins in living plant cells

Table 1 Multi-color Gateway-compatible entry clones

Fluorescent proteins	Recipient pDONR	Entry clone	att sites
Dendra	pDONR P4-P1R	pEN-L4-Dendra-R1	attB4-attB1
	pDONR221	pEN-L1-Dendra-L2	attB1-attB2
	pDONR221	pEN-L1-Dendra*-L2	attB1-attB2
	pDONR P2R-P3	pEN-R2-Dendra*-L3	attB2-attB3
Venus	pDONR P4-P1R	pEN-L4-Venus-R1	attB1-attB2
	pDONR221	pEN-L1- Venus -L2	attB1-attB2
	pDONR221	pEN-L1- Venus *-L2	attB1-attB2
	pDONR P2R-P3	pEN-R2- Venus *-L3	attB2-attB3
mCherry	pDONR P4-P1R	pEN-L4-mCherry-R1	attB4-attB1
	pDONR221	pEN-L1- mCherry -L2	attB1-attB2
	pDONR221	pEN-L1- mCherry *-L2	attB1-attB2
	pDONR P2R-P3	pEN-R2- mCherry *-L3	attB2-attB3
TagRFP	pDONR P4-P1R	pEN-L4-TagRFP-R1	attB4-attB1
	pDONR221	pEN-L1- TagRFP -L2	attB1-attB2
	pDONR221	pEN-L1- TagRFP *-L2	attB1-attB2
	pDONR P2R-P3	pEN-R2- TagRFP *-L3	attB2-attB3
Cerulean	pDONR P4-P1R	pEN-L4-Cerulean-R1	attB4-attB1
	pDONR221	pEN-L1- Cerulean -L2	attB1-attB2
	pDONR221	pEN-L1- Cerulean *-L2	attB1-attB2
	pDONR P2R-P3	pEN-R2- Cerulean *-L3	attB2-attB3

ISSN: 1746-4811