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Table 3 CBA estimates of expression variations of reference and target genes in developing Ipomoea purpurea petals across environments

From: A novel procedure for absolute real-time quantification of gene expression patterns

Variation source

Genotype

Sample size

Gene

Mean expression (transcript copies/pg cDNA)

Coefficient of Variation

From 60 HBF* until flowering

III6D

31

IpACTIN4

429.8

159%

   

IpGAPDH2

247.0

106%

   

IpDFRB-fl1

1710.5

114%

From 90 HBF until flowering

GZKL

30

IpACTIN4

133.6

80%

   

IpGAPDH2

327.1

72%

   

IpF3'H-blue

136.1

84%

   

IpWD1-a

8.8

67%

36 HBF among four days

II8II2

4

IpACTIN4

86.0

66%

   

IpGAPDH2

45.2a

27%

   

IpF3'H-blue

20.1

80%

   

IpMYB1-a

19.4

70%

 

II8SX

4

IpACTIN4

53.8

52%

   

IpGAPDH2

4.0b

113%

   

IpCHI-fl1

44.9

93%

 

S2Y6

4

IpACTIN4

39.2

95%

   

IpGAPDH2

8.1b

139%

   

IpANS-f

11.8

102%

 

YNSX

4

IpACTIN4

21.7

126%

   

IpGAPDH2

6.2b

165%

   

IpF3H-1

4.8

178%

   

Ip3GT-b

127.0

92%

Same day at four stages

SXSX2-2

4

IpACTIN4

128.6

119%

   

IpGAPDH2

258.8

124%

   

IpCHSD-us1

585.5

137%

 

SXSX2-8

4

IpACTIN4

105.1

41%

   

IpGAPDH2

238.5

40%

   

IpCHSD-mex9

474.5

65%

   

IpWD1-b

4.2

51%

  1. *HBF refers to hours before flowering. For genotypeII8II2, four days were 6, 11, 16 and 21 of September 2010. For genotypeSXSX2-2 &-8, four stages were at 9 am, 10 am, 11 am, and 12 pm of 28 September 2010.
  2. a,b Significant Wilcoxon two-sample test was indicated between superscripts, P = 0.014.