Figure 6
From: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
The release of central cytoplasmic compartments and vesicles from protoplasts under different osmotic conditions. The sucrose concentration of the cell stabilizing buffer was either undiluted (0.7 M) or diluted to 0.1, 0.2, 0.3 or 0.5 M, and the protoplasts were incubated on ice for 10 min. The number of intact protoplasts, central cytoplasmic compartments (CCC) and vacuole-derived vesicles were counted using a haemocytometer under light microscopy. The occurrence (%) is calculated from the number of protoplasts, CCC or vesicles after osmotic treatment divided by the number of protoplasts used. Each bar represents the mean value from three independent experiments ± 1 standard deviation.