Figure 2
From: Isolation of dimorphic chloroplasts from the single-cell C4 species Bienertia sinuspersici
Isolation of chlorenchyma protoplasts from B. sinuspersici. (A) A healthy branch of vegetatively propagated B. sinuspersici suitable for protoplast isolation. (B) A cross section of B. sinuspersici leaf showing chlorenchyma cells with two types of chloroplasts, i.e. the peripheral chloroplasts (P-Chls) in the peripheral cytoplasmic compartments and the central chloroplasts enclosed in the central cytoplasmic compartments (CCC). (C) A bright field image of chlorenchyma protoplasts indicating the integrity of the two cytoplasmic compartments for subsequent isolation of the dimorphic chloroplasts. (D) A bright field image of a protoplast and a vesicle with externally associated P-Chls. (E) Vital staining of isolated protoplasts imaged under epifluorescence microscopy. Fluorescein diacetate staining (left panel), chlorophyll autofluorescence (middle panel) and a merged image (right panel). Scale bars = 10 mm (A) or 20 μm (B-E).