Table 4 Mass spectrometry parameters used to quantify cytokinins, selected purines, abscisic acid and Indole-3-acetic acid
Compound | Dwell (msec) | Declustering Potential | Collision Energy | Collision Cell Exit Potential | Q1 Mass (Da) | Q3 Mass (Da) | Other Product Ions/(Abundance Rank) | Polarity |
|---|---|---|---|---|---|---|---|---|
[ 2 H 5 ](OG)Z | 10 | 116 | 29 | 20 | 387 | 206.9 | 225.2 (2),136.8 (3), 135.8 (4), 119 (5) | + |
(OG)Z | 10 | 75 | 27 | 20 | 382.2 | 220.1 | - | + |
(9G)Z | 10 | 96 | 31 | 14 | 382 | 220 | 135.9 (2), 202 (3), 148 (4), 119 (5) | + |
[ 2 H 5 ]Z | 10 | 80 | 32 | 13 | 225.1 | 136.1 | 119 (2), 207.2 (3) | + |
Z | 10 | 60 | 22 | 11 | 220.1 | 136.1 | 119 (2), 91.3 (3) | + |
[ 2 H 5 ](OG)[9R]Z | 10 | 71 | 29 | 38 | 519.1 | 386.9 | 224 (2), 207 (3), 136.7 (4) | + |
(OG)[9R]Z | 10 | 71 | 29 | 38 | 514.2 | 382.2 | - | + |
[ 2 H 5 ][9R]Z | 10 | 58 | 27 | 20 | 357.1 | 225.2 | 137 (2), 118 (3) | + |
[9R]Z | 10 | 60 | 22 | 20 | 352.1 | 220.2 | 136.1 (2), 119 (3) | + |
c Z | 10 | 60 | 22 | 11 | 220.1 | 136.1 | 119 (2), 91.3 (3) | + |
( cis )[9R]Z | 10 | 60 | 22 | 20 | 352.1 | 220.1 | 137 (2), 118 (3) | + |
[ 2 H 7 ](OG)DZ | 10 | 76 | 37 | 22 | 390.9 | 229.1 | 136 (2), 148 (3), 119 (4) | + |
(OG)DZ | 10 | 76 | 37 | 22 | 384.2 | 222.1 | - | + |
[ 2 H 3 ]DZ | 10 | 80 | 32 | 13 | 225.1 | 136.1 | 149 (2), 119.9 (3) | + |
DZ | 10 | 53 | 29 | 13 | 222.1 | 136 | 148 (2), 119 (3) | + |
[ 2 H 7 ] (OG)[9R]DZ | 10 | 71 | 30 | 28 | 523.6 | 391.2 | - | + |
(OG)[9R]DZ | 10 | 75 | 31 | 28 | 516.2 | 384.2 | - | + |
[ 2 H 3 ][9R]DZ | 10 | 58 | 27 | 20 | 357.1 | 225.2 | 135.9 | + |
[9R]DZ | 10 | 64 | 25 | 30 | 354.2 | 222.2 | 136.1 (2), 148.2 (3) | + |
[ 2 H 6 ](7G)iP | 10 | 56 | 31 | 20 | 372 | 210.1 | 137 (2), 119 (2), 74 (3) | + |
(7G)iP | 10 | 56 | 31 | 20 | 366.2 | 204.1 | - | + |
[ 2 H 6 ]iP | 10 | 60 | 22 | 13 | 210.1 | 137.1 | 148 (2), 119 (3) | + |
iP | 10 | 50 | 21 | 13 | 204.1 | 136 | 148 (2), 119 (3) | + |
[ 2 H 6 ][9R]iP | 10 | 70 | 41 | 15 | 342.1 | 137 | 210 (2), 148 (3), 119 (4) | + |
[9R]iP | 10 | 74 | 43 | 15 | 336.1 | 136 | 204 (2), 148 (3), 119 (4) | + |
[ 13 C 5 ]Ade | 10 | 96 | 35 | 26 | 140.9 | 123.8 | 95 (2). 67 (3) | + |
Ade | 10 | 96 | 31 | 26 | 136 | 119 | 91.8 (2), 64.7 (3), 108.5 (4), 66.7 (5) | + |
[ 13 C 5 ]Ado | 10 | 35 | 31 | 8 | 272.8 | 136 | 118.9 (2), 91.9 (3) | + |
Ado | 10 | 57 | 24 | 18 | 268.1 | 135.7 | 119 (2), 91.9 (3) | + |
U 15 N 5 Ino | 10 | 66 | 15 | 8 | 272.9 | 140.9 | 136 (2), 119 (3) | + |
Ino | 10 | 46 | 27 | 16 | 269.1 | 136.8 | 118.9 (2), 68.9 (3) | + |
U 15 N 5 AMP | 10 | 106 | 29 | 12 | 353 | 140.9 | 122.9 (2), 69 (3) | + |
AMP | 10 | 106 | 25 | 10 | 347.9 | 136 | 118.8 (2), 96.7 (3), 91.6 (4), 64.8 (5) | + |
[ 13 C 6 ]IAA | 10 | 40 | 30 | 10 | 180.1 | 134.2 | 143.8 (2), 119.1 (3) | - |
IAA | 10 | 43 | 26 | 10 | 174.1 | 128 | 129.7 (2) | - |
d 4 ABA | 10 | 50 | 14 | 15 | 267 | 156.1 | 223.2 (2),208.1 (3), 98.9 (4), 81.9 (5) | - |
ABA | 10 | 65 | 14 | 7 | 263 | 153.1 | 219.2 (2), 204.2 (3), 203.2 (4), 122.1 (5) | - |