Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry

Table 1 Limits of detection (LOD), limits of quantification (LLOQ), and percent relative standard deviation (%RSD) for cytokinins, purines, abscisic acid and indole-3-acetic acid

Compound	Retention Time (min)	%RSD	LOD [pM] (3s/m)	LOQ [pM] (10 s/m)
[ ² H ₅ ](OG)Z	2.09	0.24	17.71	59.05
(9G)Z	2.27	0.24	2.01	6.69
Z	2.17	0.17	4.25	14.17
[ ² H ₅ ](OG)[9R]Z	2.89	0.17	21.54	71.80
(OG)[9R]Z	3.01	0.18	-	-
[9R]Z	3.15	0.16	4.18	13.94
c Z	2.41	0.31	4.64	15.47
( *cis* )[9R]Z	3.33	0.21	3.89	12.98
[ ² H ₇ ](OG)DZ	2.26	0.26	7.39	24.65
DZ	2.22	0.22	2.13	7.11
[ ² H ₇ ] (OG)[9R]DZ	3.08	0.19	16.68	55.59
[9R]DZ	3.23	0.15	4.50	15.00
[ ² H ₆ ](7G)iP	2.88	0.19	4.69	15.62
iP	4.04	0.00	10.16	33.85
[9R]iP	4.73	4.93	6.89	22.97
IAA	2.04	0.00	742.72	2475.72
ABA	2.30	0.00	11.80	39.35
[ ¹³ C ₅ ]Ade	2.18	0.01	-	-
Ade	2.20	0.00	55.60	185.33
[ ¹³ C ₅ ]Ado	0.91	0.53	-	-
Ado	0.91	0.00	4.64	15.47
U ¹⁵ N ₅ Ino	6.42	0.06	-	-
Ino	6.42	0.08	32.54	108.47
U ¹⁵ N ₅ AMP	1.87	0.01	-	-
AMP	1.89	0.01	51.69	172.28

ISSN: 1746-4811