Figure 4
From: A simple and efficient method for the long-term preservation of plant cell suspension cultures
Temperature and phosphate effect on sucrose decrease in the nutrient medium of sycamore cell culture. Time course decrease of sucrose was measured in the nutrient medium of sycamore cell cultures at 22°C and 5°C, in the presence or absence of inorganic phosphate (Pi). Cells were initially incubated for 5 d in Pi-supplied or Pi-free NM. The cell concentration (FW) at time zero was 20 mg ml-1 culture in Pi-supplied NM and 30 mg ml-1 culture in Pi-free NM. Sucrose was quantified as described in "Material and Methods". Values are given as means ± SE (n = 3).