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Figure 6 | Plant Methods

Figure 6

From: Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Figure 6

Shoot to root reciprocal grafting. (A & B) Successfully grafted plants on BNM plates immediately before planting on Perlite. (A) The actively growing roots (indicated by red arrows) are the insert roots; the stock roots have ceased growth. (B) Yellow arrow marks root to be cut before transfer to Perlite. (C) Average nodule number on reciprocally grafted M. truncatula plants. Error bars indicate standard error of the mean. For the combinations noted as shoot/root, N = 27 for A17/A17, 4 for A17/rdn1-2, 4 for rdn1-2/A17 and 20 for rdn1-2/rdn1-2. Letters designate significant difference by Tukey-Kramer test (p < 0.05).

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Plant Methods

ISSN: 1746-4811